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OLIGONUCLEOTIDE TO BE USED IN DETERMINING SPOROGENOUS AEROBIC BACTERIUM AND DETERMINATION METHOD AND DETERMINATION KIT FOR SPOROGENOUS AEROBIC BACERIUM USING THE SAME
OLIGONUCLEOTIDE TO BE USED IN DETERMINING SPOROGENOUS AEROBIC BACTERIUM AND DETERMINATION METHOD AND DETERMINATION KIT FOR SPOROGENOUS AEROBIC BACERIUM USING THE SAME
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机译:寡核苷酸用于测定自发性好氧细菌及测定方法和试剂盒用于测定自发性好氧细菌
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摘要
An oligonucleotide which contains a base sequence specific to 16S ribosomal RNA gene of a sporogenous aerobic bacterium or a sequence complementary to the above base sequence. The specific base sequence as described above is a base sequence represented by any of SEQ ID NOS:1 to 6. This oligonucleotide is employed as a primer in a PCR reaction wherein a nucleic acid prepared from a subject bacterium to be determined is used as a specimen and the specimen serves as a template. Thus, it can be determined whether a subject bacterium is a sporogenous aerobic bacterium or not based on the amplification of a specific DNA fragment as an indication.
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机译:寡核苷酸,其含有对孢子性好氧细菌的16S核糖体RNA基因具有特异性的碱基序列或与上述碱基序列互补的序列。如上所述的特定碱基序列是由SEQ ID NOS:1至6中的任一个表示的碱基序列。该寡核苷酸在PCR反应中用作引物,其中将从待确定的对象细菌制备的核酸用作引物。标本,并以标本作为模板。因此,可以基于特定DNA片段的扩增作为指示来确定对象细菌是否是孢子性好氧细菌。
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