COCKTAIL INCUBATION LIQUID AND HIGH-THROUPHPUT SCREENING SYSTEM FOR EVALUATION OF MAJOR HUMAN CYTOCHROME P450 ENZYME ACTIVITIES AND DRUG-DRUG INTERACTIONS

摘要

The present invention uses the respective substrate drug the activity of the conventional human drug metabolism enzyme relates to a human drug-metabolizing enzyme activity measurement and drug interactions evaluation cocktail culture medium and human drug-metabolizing enzyme activity high-speed evaluation system using the same, and more particularly cocktail culture by improving that was measured in each alone (cocktail incubation) and high performance liquid chromatography-tandem mass analyzer through (LC / MS / MS) analysis cassette (cassette) with the drug candidate drug and on the market to a technique that can be searched at a high speed the possibility of drug interactions. Human drug-metabolizing enzyme activity high-speed assessment system of the present invention is important in that it reduces the time and cost required for the development of new drugs dramatically. ; Drug-metabolizing enzymes, high-performance liquid chromatograph-tandem mass spectrometer, cocktail incubation, cytochrome P450 enzymes