首页>
外国专利>
new humiliation in the mouse brain- protease and its gene
new humiliation in the mouse brain- protease and its gene
展开▼
机译:小鼠脑中新的羞辱蛋白酶及其基因
展开▼
页面导航
摘要
著录项
相似文献
摘要
PURPOSE: SUMO-specific protease, mUlpB from mouse brain and gene thereof are provided, which are useful for development of anticancer drugs including acute leukemia. CONSTITUTION: The SUMO-specific protease, mUlpB is isolated from mouse brain, wherein the SUMO-specific protease digests protein or peptide bound to SUMO; the SUMO-specific protease has the total or portion of the amino acid sequence of SEQ ID NO:2; and the SUMO-specific protease has molecular weight of 55 kDa. The SUMO-specific protease gene has the total or portion of the nucleotide sequence of SEQ ID NO:1. The expression plasmids pET32a-mUIpB and pCMV-Tag-mUIpB contain the total or portion of the nucleotide sequence of SEQ ID NO:1. A transformant is produced by transforming microorganism BL21 with the expression plasmid pET32a-mUIpB.
展开▼
机译:目的:提供SUMO特异性蛋白酶,来自小鼠脑的mUlpB及其基因,其可用于开发包括急性白血病在内的抗癌药物。组成:SUMO特异性蛋白酶mUlpB是从小鼠脑中分离得到的,其中SUMO特异性蛋白酶消化与SUMO结合的蛋白质或肽。 SUMO-特异性蛋白酶具有SEQ ID NO:2的全部或部分氨基酸序列; SUMO特异性蛋白酶的分子量为55kDa。 SUMO特异性蛋白酶基因具有SEQ ID NO:1的核苷酸序列的全部或部分。表达质粒pET32a-mUIpB和pCMV-Tag-mUIpB包含SEQ ID NO:1的核苷酸序列的全部或部分。通过用表达质粒pET32a-mUIpB转化微生物BL21来产生转化体。
展开▼