SCREENING SYSTEM FOR ANTHRACNOSE RESISTANCE IN CAPSICUM SPP. USING IN PLANTA MICROINJECTION METHOD

摘要

The present invention relates to the development of a new resistance assay method for pepper anthrax, which is inoculated using a microinjection method on a plant in a natural packaging state without harvesting red pepper fruits, and then includes a plastic glove containing a wet towel. It is sealed to maintain saturated relative humidity for 48 hours, and then to investigate resistance or pathogenic reaction after 7 days of sealing. The newly developed method is the existing method, the Detached microinjection method, that is, the fruit is harvested from plants, washed, inoculated in-flight and placed in an artificially controlled artificial culture room to investigate the disease after 7 days. There was no difference in pathogenicity compared to the above, and by reducing the harvesting and washing stages of the fruits, labor can be saved and, above all, the reaction in the natural pepper redistribution environment rather than the virtual cabin environment is more practical. It was judged how. In addition, the development of the pavement resistance test method, which is a serious problem in the method of investigating the development of pavement resistance, that is, in the natural state without artificial inoculation, and selecting and evaluating relatives between individuals and strains, has been considered as a serious problem. It was thought to be a groundbreaking way to reduce escape. In other words, the diseased fruit continuously develops in the state of being attached to the plant and exists in the package, which plays the role of a secondary infectious agent, thus maintaining the density of pathogens in the field and finally packing in the late natural state. Also in resistance evaluation, it is an important means to raise the selection pressure or the selection strength. Finally, in the selection of strains and individuals for the growth of red pepper anthrax resistant varieties, the present invention was first assayed using the micro-injection method in the plant, and secondly, the micro-injection method was harvested, and finally the packaging resistance at the later stage of cultivation. By screening for, we have established a new triple screening system that enables stable resistance lines and breeding by minimizing the avoidance of onset or selection errors.;Pepper Anthrax, Resistance Assay, In Planta Microinjection, Detached Microinjection, Pavement Resistance, Triple Screening System