SCREENING SYSTEM FOR ANTHRACNOSE RESISTANCE IN CAPSICUM SPP. USING IN PLANTA MICROINJECTION METHOD

摘要

invention pepper anthracnose resistance test method for new development in pepper fruits of the harvest to be written without microinjection into the state with the plant in its natural packaging (microinjection method) inoculated using a saturated relative humidity was maintained 48 hours, sealed with plastic gloves that contain the wet towel and then 7 days after pibong Susceptible resistance or reaction a method of irradiating. The method developed a new way to investigate the outbreak 7 days after you place the conventional method of harvest after microinjection (Detached microinjection method), ie the controlled growth chamber artificial inoculation and then the environment in the cabin after washing the fruits harvested from plants Compared to develop practical skills, the differences are more in terms that were not able to save labor by reducing the harvesting and washing steps of the fruit, and above all, a virtual cabin environment is not to determine the natural environment under pepper cultivation packaging reaction How was judged. Also been carried out on the existing pavement resistance test method, ie the difference between intestinal disease due to environmental and redistribution that has been emerging as a serious problem in how to investigate the outbreak situations in nature, without artificial inoculation and selection between the object and the relative rating system to reduce evasion (escape) was considered revolutionary way. That is infected with the packing of fruit and finally grown late onset natural state, thus is constantly in progress and the state with the packing, which exists in the zoom maintain a high packing density of the pathogen by performing a role of secondary plant jeonyeomwon an important tool that can increase the selection pressure for resistance or starting strength is also evaluated. Finally, using the plant body, primarily in the microinjection system and object pepper anthracnose resistant varieties selected for training by the present invention after harvest as black and black as a secondary microinjection then finally packing resistant to late planting The test was able to establish a reliable method of enabling a new three-resistant strains and varieties upbringing by avoiding or minimizing the error in the selection of cases (triple screening system) and by black.