Fluorescence Activated Cell SortingFACS Apparatus with Laser Diodes for Characterizing and Sorting Cells and Method For Sorting the Cells Using the Same

摘要

The present invention relates to a fluorescence activated cell sorting apparatus for detecting and classifying biological cells using a laser diode. It consists of a cell detection continuous operation laser diode which detects the flow of the cell passing through a detection area, and a pulse operation laser diode which emits an optical pulse for a predetermined time at the time when a cell is detected. Since the fluorescent active cell sorting apparatus of the present invention uses a laser diode, it is relatively small in size, light in weight, low power consumption, and low in cost compared with the conventional fluorescent active cell sorting apparatus using an argon ion laser. Can be. In addition, the method for classifying cells according to the present invention detects the flow of cells by shining light from the cell detection continuous operation laser diode to the detection region, and the high power light pulse from the pulsed laser diode is reflected on the detected cells to Scattered light or fluorescence emitted from the light is detected, and the characteristics of the cells are analyzed and classified based on the scattered light signal or the fluorescence signal converted from the scattered light and the fluorescence.;Biological Cells, Fluorescent Active Cells, FACS, Laser Diode, Cell Detection Continuous Operation Laser Diode, Pulsed Laser Diode, Cell Classification