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Method of constructing a model of a cellular development and differentiation with use of systems of homozygous stem cells, a method of estimating and cataloging EXPRESSED of protein, obtained from these cDNA libraries, AND MATERIALS AND METHODS FOR USING THEM
Method of constructing a model of a cellular development and differentiation with use of systems of homozygous stem cells, a method of estimating and cataloging EXPRESSED of protein, obtained from these cDNA libraries, AND MATERIALS AND METHODS FOR USING THEM
1. method u043au043eu043du0441u0442u0440u0443u0438u0440u043eu0432u0430u043du0438u00a0 model cellular u0440u0430u0437u0432u0438u0442u0438u00a0 and differentiation using u0433u043eu043cu043eu0437u0438u0433u043eu0442u043du044bu0445 stem cells derived from the donor material, called u043cu0430u0442u0440u0438u0432u0430u044eu0449u0438u0439 stage;(a) u043fu043eu043bu0443u0447u0435u043du0438u00a0 allocated hs cells.;(b) u043fu043eu043bu0443u0447u0435u043du0438u00a0 desired cells, groups of cells or tissue type u043du0430u0446u0435u043bu0438u0432u0430u043du0438u0435u043c allocated hs cells and the differentiation;(c) periodically samples the u0432u0437u00a0u0442u0438u00a0 allocated hs cells, u043fu043eu0434u0432u0435u0440u0433u0430u044eu0449u0438u0445u0441u00a0 aimed u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0435.;2. method for sample 1, where the periodic u0432u0437u00a0u0442u0438u0435 allocated hs cells, u043fu043eu0434u0432u0435u0440u0433u0430u044eu0449u0438u0445u0441u00a0 aimed u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0435 provides;(a) the periodic extractions of cellular rna and then the messenger rna of the hs cells, u043fu043eu0434u0432u0435u0440u0433u0430u044eu0449u0438u0445u0441u00a0 aimed u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0435; and;(b) the design of the library u043au0434u043du043a focal messenger rna.;3. way to 1, where the hs cell stage (a) is obtained from a donor u0437u0430u0440u043eu0434u044bu0448u0435u0432u043eu0433u043e material through;(a) u043fu043eu043bu0443u0447u0435u043du0438u00a0 u043cu0438u0442u043eu0442u0438u0447u0435u0441u043au0438 u0430u043au0442u0438u0432u0438u0440u043eu0432u0430u043du043du043eu0439 u0433u043eu043cu043eu0437u0438u0433u043eu0442u043du043eu0439 i. embryonic cells post u043cu0435u0439u043eu0437u0430 i: u0441u043bu0438u00a0u043du0438u0435u043c two u043eu043eu0446u0438u0442u043eu0432 or two u0441u043fu0435u0440u043cu0430u0442u0438u0434 axt, prevention the second u043fu043eu043bu00a0u0440u043du043eu0433u043e u0440u0443u0437u0438u0438 body in u0432u0440u0435u043cu00a0 u043eu043eu0433u0435u043du0435u0437u0430,the second u043fu043eu043bu00a0u0440u043du043eu0433u043e u043fu043eu0437u0432u043eu043bu0435u043du0438u0435u043c extrusion body and spontaneous genome u0441u0430u043cu043eu0440u0435u043fu043bu0438u043au0430u0446u0438u0438 in u043fu043eu0434u0445u043eu0434u00a0u0449u0438u0445 u0443u0441u043bu043eu0432u0438u00a0u0445 or transfer of two u00a0u0434u0435u0440 sperm or two u00a0u0434u0435u0440 mr. u0430u043fu043bu043eu0438u0434u043du043eu0439 u00a0u0439u0446u0435u043au043bu0435u0442u043au0438 in u044du043du0443u043au043bu0435u0438u0440u043eu0432u0430u043du043du044bu0439 u043eu043eu0446u0438u0442;;(b) the u0430u043au0442u0438u0432u0438u0440u043eu0432u0430u043du043du043eu0439 u043au0443u043bu044cu0442u0438u0432u0438u0440u043eu0432u0430u043du0438u00a0 u0433u043eu043cu043eu0437u0438u0433u043eu0442u043du043eu0439 i. embryonic cells post u043cu0435u0439u043eu0437u0430 i u0434u043bu00a0 u043eu0431u0440u0430u0437u043eu0432u0430u043du0438u00a0 such u0431u043bu0430u0441u0442u043eu0446u0438u0441u0442u0435 mass and;(c) u0432u044bu0434u0435u043bu0435u043du0438u00a0 u0433u043eu043cu043eu0437u0438u0433u043eu0442u043du044bu0445 stem cells from the inner cell mass (u044du043cu0431u0440u0438u043eu0431u043bu0430u0441u0442u0430) indicated that u0431u043bu0430u0441u0442u043eu0446u0438u0441u0442u0435 mass;and when u043cu0438u0442u043eu0442u0438u0447u0435u0441u043au0438 u0430u043au0442u0438u0432u0438u0440u043eu0432u0430u043du043du0430u00a0 u0433u043eu043cu043eu0437u0438u0433u043eu0442u043du0430u00a0 u0434u0438u043fu043bu043eu0438u0434u043du0430u00a0 u0437u0430u0440u043eu0434u044bu0448u0435u0432u0430u00a0 cell post u043cu0435u0439u043eu0437u0430 i u043eu0431u0440u0430u0437u0443u0435u0442u0441u00a0 u0441u043bu0438u00a0u043du0438u0435u043c two u043eu043eu0446u0438u0442u043eu0432 or two u0441u043fu0435u0440u043cu0430u0442u0438u0434 or u043fu0435u0440u0435u043du043e two u00a0u0434u0435u0440 catfish sperm or two u00a0u0434u0435u0440 u0433u0430u043fu043bu043eu0438u0434u043du043eu0439 u00a0u0439u0446u0435u043au043bu0435u0442u043au0438 in u044du043du0443u043au043bu0435u0438u0440u043eu0432u0430u043du043du044bu0439 u043eu043eu0446u0438u0442, u0433u043eu043cu043eu0437u0438u0433u043eu0442u043du043eu0441u0442u044c identified stem cells confirmed the u0433u0435u043du043eu0442u0438u043fu0438u0440u043eu0432u0430u043d u0438u0435u043c.;4. method for u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0430 stage 1, where (b) u0432u044bu043fu043eu043bu043du00a0u0435u0442u0441u00a0 in vitro incorporation of regulatory factors, hormone or u0446u0438u0442u043eu043au0438u043du0430 cells in u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0443u044e wednesday.;5. method for u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0430 stage 1, where (b) u0432u044bu043fu043eu043bu043du00a0u0435u0442u0441u00a0 in vivo and for hs cells in transplant kidney capsule, u0431u0440u044eu0448u0438u043du043du0443u044e cavity or percutaneously.;6. method for u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0430 stage 1, where (b) u0432u044bu043fu043eu043bu043du00a0u0435u0442u0441u00a0 using three-dimensional u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 system for supporting cells preceding harvest u0441u0442u0432u0435u043du043du0438u043au043eu0432 who u00a0u0432u043bu00a0u044eu0442u0441u00a0 u0442u043au0430u043du0435u0441u043fu0435u0446u0438u0444u0438u0447u0435u0441u043au0438u043cu0438, u043du0435u0439u043bu043eu043du043eu0432u0443u044e grid, and then the nylon nets u0438u043du043eu043au0443u043bu00a0u0446u0438u044e fresh or u043au0440u0438u043eu043au043eu043du0441u0435u0440u0432u0438u0440u043eu0432u0430u043du043du044bu043cu0438 hs cells and.;7. method for u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0430 stage 1, where (b) u0432u044bu043fu043eu043bu043du00a0u0435u0442u0441u00a0 using three-dimensional u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 system consisting of;(a) the seed cells in the grid, and u0431u0438u043eu0434u0435u0433u0440u0430u0434u0438u0440u0443u0435u043cu0443u044e hs;(b) the transplant of the grid in the host or u043cu0438u043au0440u043eu043eu043au0440u0443u0436u0435u043du0438u0435 containing cell u0440u0435u0433u0443u043bu00a0u0442u043eu0440u044b growth hormones and / or cytokine.;8. way on p.7, where the u043cu0438u043au0440u043eu043eu043au0440u0443u0436u0435u043du0438u0435 u00a0u0432u043bu00a0u0435u0442u0441u00a0 capsule kidney.;9. way on p.7, where the u043cu0438u043au0440u043eu043eu043au0440u0443u0436u0435u043du0438u0435 u00a0u0432u043bu00a0u0435u0442u0441u00a0 u0431u0440u044eu0448u0438u043du043du043eu0439 complete.;10. way on p.7, where the u043cu0438u043au0440u043eu043eu043au0440u0443u0436u0435u043du0438u0435 u00a0u0432u043bu00a0u0435u0442u0441u00a0 hs cells, u0432u044bu0440u0430u0449u0435u043du043du044bu043cu0438 with elements of different tissues in vitro.;11. way on p.7, where the host u00a0u0432u043bu00a0u0435u0442u0441u00a0 naked mouse.;12. method for u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0430 stage 1, where (b) u0432u044bu043fu043eu043bu043du00a0u0435u0442u0441u00a0 in vivo and in vitro, which;(a) the transplant hs cells in u043cu0438u043au0440u043eu043eu043au0440u0443u0436u0435u043du0438u0435;;(b) the cell precursors from the u043cu0438u043au0440u043eu043eu043au0440u0443u0436u0435u043du0438u00a0 and;(c) additional u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0443 specified cell precursors in vitro.;13. way to 1, where the u0432u0437u00a0u0442u0438u0435 sampling stage (c) provides for the collection of hs cells at different u0441u0442u0430u0434u0438u00a0u0445 differentiation in vitro.;14. way to 1, where the u0432u0437u00a0u0442u0438u0435 sampling stage (c) provides for the microscopic identification and u043cu0438u043au0440u043eu0434u0438u0441u0441u0435u043au0446u0438u044e hs cells at different u0441u0442u0430u0434u0438u00a0u0445 differentiation in vivo or in vitro differentiation in three-dimensional culture.;15. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 u043au0435u0440u0430u0442u0438u043du0438u0437u0438u0440u0443u044eu0449u0438u0435u0441u00a0 epithelial cells.;16. method for u043au0435u0440u0430u0442u0438u043du0438u0437u0438u0440u0443u044eu0449u0438u0435u0441u00a0 p.15, where the epithelial cells selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of u043au0435u0440u0430u0442u0438u043du043eu0446u0438u0442u043eu0432 epidermis basal cells of epidermis, u043au0435u0440u0430u0442 u0438u043du043eu0446u0438u0442u043eu0432 nails of fingers of hands and nails toes, leading the basal cells (cells u0432u043eu043bu043eu0441u00a0u043du043eu0433u043e u0441u0442u0435u0440u0436u043du00a0, u044du043fu0438u0442u0435u043bu0438u0430u043bu044cu043du043eu0433u043e u043au043eu0440u043du00a0 hair and vaginal cells u043cu0430u0442u0440 x's hair.;17. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 cells u044du043fu0438u0442u0435u043bu0438u0435u0432 humid layer barriers.;18. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 epithelial cells, specialized u0434u043bu00a0 u044du043au0437u043eu043au0440u0438u043du0430 secretion.;19. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 epithelial cells, specialized u0434u043bu00a0 secretion of hormones.;20. method for group 1, where the desired u00a0u0432u043bu00a0u044eu0442u0441u00a0 intestinal epithelial cells the absorbing cells, u044du043au0437u043eu043au0440u0438u043du043du044bu0445 glands and u043cu043eu0447u0435u043fu043eu043bu043eu0432u044bu0445 ways.;21. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 cells specialized u0434u043bu00a0 metabolism and u0437u0430u043fu0430u0441u0430u043du0438u00a0.;22. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 barrier epithelial cells that u0432u044bu0441u0442u0438u043bu0430u044eu0442 lungs, intestines, u044du043au0437u043eu043au0440u0438u043du043du044bu0435 glands and u043cu043eu0447u0435u043fu043eu043bu043eu0432u044bu0435 way.;23. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 epithelial cells, u0432u044bu0441u0442u0438u043bu0430u044eu0449u0438u0435 confined inside the body cavity.;24. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 u0446u0438u043bu0438u0430u0440u043du044bu0435 (with cilium) cells with u043fu0440u043eu043fu0443u043bu044cu0441u0438u043eu043du043du043eu0439 (u0432u044bu0442u0430u043bu043au0438u0432u0430u044eu0449u0435u0439) function.;25. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 cells specialized u0434u043bu00a0 of u0432u043du0435u043au043bu0435u0442u043eu0447u043du043eu0433u043e u043cu0430u0442u0440u0438u043au0441u0430.;26. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 u0441u043eu043au0440u0430u0442u0438u0442u0435u043bu044cu043du044bu0435 cells.;27. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 blood cells and immune system.;28. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 sensor u0442u0440u0430u043du0441u0434u0443u043au0442u043eu0440u044b.;29. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 neurons u043eu0442u043du043eu0441u00a0u0449u0438u0435u0441u00a0 to the autonomic nervous system.;30. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 supporting cells and peripheral sensory neurons.;31. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 cells of the central nervous system, including u0441u0435u0431u00a0 neurons and u0433u043bu0438u0430u043bu044cu043du044bu0435 cells.;32. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 lens cells.;33. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 pigmented cells.;34. method for group 1, where the desired cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 sexual cells.;35. method for group 1, where the desired u00a0u0432u043bu00a0u044eu0442u0441u00a0 u0441u0443u0441u0442u0435u043du043eu0446u0438u0442u044b cells (supporting cells u00a0u0438u0447u043au0430).;36. u0432u044bu0434u0435u043bu0435u043du043du0430u00a0 messenger rna, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 nucleotide sequences for various u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u044b where the messenger rna u044du043au0441u0442u0440u0430u0433u0438u0440u0443u044eu0442 and u0432u044bu0434u0435u043bu00a0u044eu0442 in u043fu043eu0434u0445u043eu0434u00a0u0449u0438u0445 u0438u043du0442u0435u0440u0432 u0430u043bu0430u0445 allocated time of hs cells at different u0441u0442u0430u0434u0438u00a0u0445 focused differentiation in vivo and / or in vitro.;37. messenger rna for p.36, where the u043du0443u043au043bu0435u043eu0442u0438u0434u043du044bu043c u043fu043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u00a0u043c precedes u0444u0443u043du043au0446u0438u043eu043du0430u043bu044cu043du0430u00a0 u043fu0440u043eu043cu043eu0442u043eu0440u043du0430u00a0 sequence u0440u0430u0441u043fu043eu043bu043eu0436u0435u043du043du0430u00a0 5 '(left) on the cl u0430u0437u0430u043du043du043eu0439 sequence.;38. messenger rna for p.36, where at least one u043au043eu043fu0438u00a0 the nucleotide sequences present in u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du043eu043c rna vector.;39. u0432u044bu0434u0435u043bu0435u043du043du0430u00a0 u043au0434u043du043a, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 nucleotide sequences, u043au043eu043cu043fu043bu0438u043cu0435u043du0442u0430u0440u043du044bu0435 focal messenger rna, where the messenger rna u044du043au0441u0442u0440u0430u0433u0438u0440u0443u044eu0442 and u0432u044bu0434u0435u043bu00a0u044eu0442 in u043fu043eu0434u0445u043eu0434u00a0u0449u0438u0445 u0438u043du0442u0435u0440u0432u0430 lah time identified hs cells at different u0441u0442u0430u0434u0438u00a0u0445 focused differentiation in vivo and / or in vitro.;40. u043au0434u043du043a to p.39, where the u043du0443u043au043bu0435u043eu0442u0438u0434u043du044bu043c u043fu043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u00a0u043c precedes u0444u0443u043du043au0446u0438u043eu043du0430u043bu044cu043du0430u00a0 u043fu0440u043eu043cu043eu0442u043eu0440u043du0430u00a0 sequence u0440u0430u0441u043fu043eu043bu043eu0436u0435u043du043du0430u00a0 5 '(left) on the cl u0430u0437u0430u043du043du043eu0439 sequence.;41. u043au0434u043du043a to p.39, where at least one u043au043eu043fu0438u00a0 the nucleotide sequences present in u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du043eu043c dna vector.;42. the u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u044b, u043au043eu043cu043fu043bu0438u043cu0435u043du0442u0430u0440u043du044bu0435 messenger rna, where the messenger rna u044du043au0441u0442u0440u0430u0433u0438u0440u0443u044eu0442 and u0432u044bu0434u0435u043bu00a0u044eu0442 identified hs cells at different u0441u0442u0430u0434u0438u00a0u0445 aimed u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440 u043eu0432u043au0438, and where the dedicated u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u044b contain at least 10 consecutive nucleotides having at least 65% by u0433u043eu043cu043eu043bu043eu0433u0438u044e on the messenger rna.;43. the u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u044b, u043au043eu043cu043fu043bu0438u043cu0435u043du0442u0430u0440u043du044bu0435 u043au0434u043du043a where u0443u043au0430u0437u0430u043du043du0430u00a0 u043au0434u043du043a u043au043eu043cu043fu043bu0435u043cu0435u043du0442u0430u0440u043du0430 messenger rna molecules, which u044du043au0441u0442u0440u0430u0433u0438u0440u0443u044eu0442 and u0432u044bu0434u0435u043bu00a0u044eu0442 identified hs cells in ra u0437u043bu0438u0447u043du044bu0445 u0441u0442u0430u0434u0438u00a0u0445 focused differentiation, and where the dedicated u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u044b contain at least 10 consecutive nucleotides having, on me u044cu0448u0435u0439 as 65% of their u0433u043eu043cu043eu043bu043eu0433u0438u044e on the u043au0434u043du043a.;44. the u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u044b on p.42 and 43, where the u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u044b marked u0434u0435u0442u0435u043au0442u0438u0440u0443u0435u043cu043eu0439 label.;45. the u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u044b peptides, and proteins encoded u044du043au0441u0442u0440u0430u0433u0438u0440u043eu0432u0430u043du043du043eu0439 messenger rna, and extracted from the allocated hs cells at different u0441u0442u0430u0434u0438u00a0u0445 focused differentiation.;46. method of identification of genetic material u043au043eu0434u0438u0440u0443u044eu0449u0435u0433u043e various genes involved in u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0435 allocated hs cells into the desired cell or group of cells tissue type with;(a) the provision of genetic material from u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u0443u044eu0449u0438u0445u0441u00a0 hs cells u0434u043bu00a0 u043fu043eu043bu0443u0447u0435u043du0438u00a0 samples of genetic material;(b) u043au043eu043du0442u0430u043au0442u0438u0440u043eu0432u0430u043du0438u0435 the samples with u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u043eu043c in u0443u0441u043bu043eu0432u0438u00a0u0445 hybridization and;(c) detection of u043eu0431u0440u0430u0437u043eu0432u0430u043du0438u00a0 duplex containing the oligonucleotide and the genetic material present in the sample.;47. method u0438u0441u043fu044bu0442u0430u043du0438u00a0 u0434u0435u0439u0441u0442u0432u0438u00a0 stimulus for cellular u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0443 using systems u0433u043eu043cu043eu0437u0438u0433u043eu0442u043du044bu0445 stem (hs) cells derived from the donor material, etc. u0435u0434u0443u0441u043cu0430u0442u0440u0438u0432u0430u044eu0449u0438u0439;(a) u043au043eu043du0442u0430u043au0442u0438u0440u043eu0432u0430u043du0438u0435 incentive with the selected hs cells at different differentiation and u0441u0442u0430u0434u0438u00a0u0445 aimed;(b) u0432u0437u00a0u0442u0438u0435 samples of the hs cells at various intervals.;48. method u0434u0435u0442u0435u043au0442u0438u0440u043eu0432u0430u043du0438u00a0 u0434u0435u0439u0441u0442u0432u0438u00a0 stimulus for cellular u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0443 through u043au043eu043du0442u0430u043au0442u0438u0440u043eu0432u0430u043du0438u00a0 the incentive to u0433u043eu043cu043eu0437u0438u0433u043eu0442u043du044bu043cu0438 stem (hs) cells, paul u0443u0447u0435u043du043du044bu043cu0438 of donor material for;(a) the periodic extractions of cellular rna of hs cells, u043du0430u0445u043eu0434u00a0u0449u0438u0445u0441u00a0 in contact with the catalyst.;(b) allocation of the cellular rna and messenger rna;(c) the design of the library u043au0434u043du043a focal messenger rna.;49. method u043eu0431u0435u0441u043fu0435u0447u0435u043du0438u00a0 electronic database of sequences of genes and / or proteins involved in cellular development and u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0435 u0433u043eu043cu043eu0437u0438u0433u043eu0442u043du044bu0445 stem tc u0435u0442u043eu043a for;(a) information on the u043fu043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u00a0u0445 genes and proteins u0434u043bu00a0 u0445u0440u0430u043du0435u043du0438u00a0 in a remote location u0434u043bu00a0 subsequent search and retrieval.;(b) to customize the u0442u0440u0435u0431u043eu0432u0430u043du0438u00a0 u043fu043eu043bu044cu0437u043eu0432u0430u0442u0435u043bu00a0 search in the local computer using the input key u043fu043eu043bu00a0;;(c) transfers the search stage (b) in a remote location and the sample matches containing the sequences of genes and proteins, u0441u043eu043eu0442u0432u0435u0442u0441u0442u0432 u0443u044eu0449u0438u0445 the search in the limit errors; and;(d) provision of information on u0441u043eu0432u043fu0430u0434u0435u043du0438u00a0u0445 in graphics and u043du0430u0433u043bu00a0u0434u043du043eu043c format.;50. the database system based on the internet u0434u043bu00a0 sequences of genes and / or proteins involved in cellular development and u0434u0438u0444u0444u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0435 u0433u043eu043cu043eu0437u0438u0433u043eu0442u043du044bu0445 stem tc u0435u0442u043eu043a, u0441u043eu0434u0435u0440u0436u0430u0449u0430;(a) funds u0434u043bu00a0 access and u0445u0440u0430u043du0435u043du0438u00a0 information;;(b) the means of u0434u043bu00a0 u0444u043eu0440u043cu0443u043bu0438u0440u043eu0432u0430u043du0438u00a0 customize under u0442u0440u0435u0431u043eu0432u0430u043du0438u00a0 u043fu043eu043bu044cu0437u043eu0432u0430u0442u0435u043bu00a0 search query.;(c) the means of u0434u043bu00a0 u0438u0441u043fu043eu043bu043du0435u043du0438u00a0 customize under u0442u0440u0435u0431u043eu0432u0430u043du0438u00a0 u043fu043eu043bu044cu0437u043eu0432u0430u0442u0435u043bu00a0 search query and;(d) funds u0434u043bu00a0 return results of the search in the u043du0430u0433u043bu00a0u0434u043du043eu0439 and graphic design; where a search query may u0432u044bu043fu043eu043bu043du00a0u0442u044cu0441u00a0 many tc u044eu0447u0435u0432u044bu0445 fields.;51. u0438u0441u043au0443u0441u0441u0442u0432u0435u043du043du0430u00a0 system u043fu043eu0434u0434u0435u0440u0436u0430u043du0438u00a0 bodies u043au043eu0442u043eu0440u0430u00a0 contains;(a) as the layer containing the tissue, u0438u043cu043cu043eu0431u0438u043bu0438u0437u043eu0432u0430u043du043du044bu0435 on u043cu0430u043au0440u043eu043fu043eu0440u0438u0441u0442u043eu043c medium, where the tissue through which u043au0443u043bu044cu0442u0438u0432u0438u0440u0443u044eu0442u0441u00a0 diff u0435u0440u0435u043du0446u0438u0440u043eu0432u043au0438 allocated hs cells and are capable of long-term proliferation; and;(b) u043au043eu043du0434u0438u0446u0438u043eu043du0438u0440u0443u044eu0449u0438u0439 vessel attached to the protected layer, where the environment u0434u043bu00a0 u0432u044bu0440u0430u0449u0438u0432u0430u043du0438u00a0 u043fu043eu0434u0430u0435u0442u0441u00a0 pump from the receptacle to have air conditioning u043au0430u0437u0430u043du043du043eu043cu0443 contained layer and back.;52. u0438u0441u043au0443u0441u0441u0442u0432u0435u043du043du0430u00a0 system u043fu043eu0434u0434u0435u0440u0436u0430u043du0438u00a0 p.51 bodies, where the body u00a0u0432u043bu00a0u0435u0442u0441u00a0 kidney and the tissue selected from the group u0441u043eu0441u0442u043eu00a0u0449u0435u0439 of u043fu0430u0440u0438u0435u0442u0430u043bu044cu043du044bu0445 cleto to u043fu043eu0434u043eu0446u0438u0442u043eu0432 kidney u043au043bu0443u0431u043eu0447u043au0430, thin segment loop henle cells, renal cells u043au0430u043du0430u043bu044cu0446u0435u0432, cells u043eu043au043eu043bu043eu0433u043bu043eu043cu0435u0440u0443u043bu00a0u0440u043du043eu0433u043e apparatus cells u0449u0435u0442u043eu0447u043du043eu0439 margins about u043au0441u0438u043cu0430u043bu044cu043du043eu0433u043e kidney u043au0430u043du0430u043bu044cu0446u0430 and cells of distal kidney u043au0430u043du0430u043bu044cu0446u0430.;53. u0438u0441u043au0443u0441u0441u0442u0432u0435u043du043du0430u00a0 system u043fu043eu0434u0434u0435u0440u0436u0430u043du0438u00a0 bodies u00a0u0432u043bu00a0u0435u0442u0441u00a0 p.51, where the body of pancreas, and the tissue selected from the group of al, u0441u043eu0441u0442u043eu00a0u0449u0435u0439 f -, beta - and delta - cells of islets of langerhans.;54. u0438u0441u043au0443u0441u0441u0442u0432u0435u043du043du0430u00a0 system u043fu043eu0434u0434u0435u0440u0436u0430u043du0438u00a0 p.51 bodies, where the body u00a0u0432u043bu00a0u0435u0442u0441u00a0 liver, and the liver cells u00a0u0432u043bu00a0u044eu0442u0441u00a0 u0433u0435u043fu0430u0442u043eu0446u0438u0442u0430u043cu0438.
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