Selective culture medium for the isolation and identification of species of the genus STREPTOCOCCUS

摘要

1.u0441u0435u043bu0435u043au0442u0438u0432u043du0430u00a0 u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0430u00a0 wednesday u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 and early u0432u044bu00a0u0432u043bu0435u043du0438u00a0 species of genus streptococcus, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0 in that it contains a group of nourishing nutrients the framework of from 15 to 58 g / l, which provide a total nitrogen content of essentially of proteins, from 10 to 14%, selected from u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442u0430 bovine blood bovine heart extract.u0444u0435u0440u043cu0435u043du0442u0430u0442u0438u0432u043du043eu0433u043e u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442u0430 bovine cardiac muscle, u0444u0435u0440u043cu0435u043du0442u0430u0442u0438u0432u043du043eu0433u043e u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442u0430 protein milk, u0444u0435u0440u043cu0435u043du0442u0430u0442u0438u0432u043du043eu0433u043e u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442u0430 proteins of soybeans, u0444u0435u0440u043cu0435u043du0442u0430u0442u0438u0432u043du043eu0433 on the u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442u0430 animal tissue, u0430u0443u0442u043eu043bu0438u0437u0430u0442u0430 or u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442u0430 yeast Saccharomyces cerevisiae and their mixtures; the group of inhibitors in quantity from 0.55 to 16 g / l in the case of u0441u043eu0435u0434u0438u043du0435u043du0438u00a0 acetate u0442u0430u043bu0438u00a0 and u043du0430u043bu0438u0434u0438u043au0441u043eu0432u043eu0439 acid; a mixture of u0434u0435u0433u0440u0430u0434u0438u0440u0443u0435u043cu044bu0445 u0445u0440u043eu043cu043eu0433u0435u043du043du044bu0445 and u0444u043bu0443u043eu0440u043eu0433u0435u043du043du044bu0445 substances, at least u0434u043bu00a0 enzyme for each species the number of from 0.3 to 1.5 g / l, chosen from a u043du0438u0442u0440u043eu0444u0435u043du0438u043bu0444u043eu0441u0444u0430u0442u043du043eu0439 salt u0434u0438u043du0430u0442u0440u0438u00a0; 5 - bromine - 4 - chlorine - 3 - u0438u043du0434u043eu043bu0438u043b - d - u0433u043bu044eu043au043eu043fu0438u0440u0430u043du043eu0437u0438u0434u0430, 4 - u043cu0435u0442u0438u043bu0443u043cu0431u0435u043bu043bu0438u0444u0435u0440u0438u043b - - d - u0433u043bu044eu043au043eu043fu0438u0440u0430u043du043eu0437u0438u0434u0430 (6 - u0445u043b or 3 - u0438u043du0434u043eu043bu0438u043b - - d - u0433u043bu044eu043au043eu043fu0438u0440u0430u043du043eu0437u0438u0434u0430)u0446u0438u043au043bu043eu0433u0435u043au0441u0438u043bu0430u043cu043cu043eu043du0438u0435u0432u043eu0439 salt of 5 - bromine - 4 - chlorine - 3 - u0438u043du0434u043eu043bu0438u043b - - d - u0433u043bu044eu043au0443u0440u043eu043du0438u0434u0430, u0446u0438u043au043bu043eu0433u0435u043au0441u0438u043bu0430u043cu043cu043eu043du0438u0435u0432u043eu0439 salt of 5 - bromine - 6 - chlorine - 3 - u0438u043du0434u043eu043bu0438u043b - - d - u0433u043bu044eu043au0443u0440u043eu043du0438u0434u0430 and u0442u0440u0438u0433u0438u0434u0440u0430u0442u0430 4 u043cu0435u0442u0438u043bu0443u043cu0431u0435u043bu043bu0438u0444u0435u0440u0438u043b - - d - u0433u043bu044eu043au0443u0440u043eu043du0438u0434u0430 and u0441u043eu0435u0434u0438u043du0435u043du0438u00a0, u00a0u0432u043bu00a0u044eu0449u0438u0435u0441u00a0 indicators u0434u0435u0437u0430u043cu0438u043du0430u0437u043du043eu0439 activity, in the amount of from 1 to 3 g / l.;2. u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0430u00a0 wednesday on p.1, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0 because it contains nutritious framework established in the following quantities in the environment: u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442 bovine blood 5 to 10 g / l bovine heart extract 1 - 12 g / l bovine cardiac muscle enzyme u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442 1 to 5 g / l, the enzyme u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442 proteins of milk with 5 to 20 g / l, the enzyme u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442 proteins of soybeans 2 to 15 g / l enzyme u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442 animal tissue 1 to 5 g / lu0430u0443u0442u043eu043bu0438u0437u0430u0442 or u0433u0438u0434u0440u043eu043bu0438u0437u0430u0442 yeast Saccharomyces cerevisiae 2 to 15 g / l.;3. u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0430u00a0 environment 1, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0 because it contains inhibitors of growth u0433u0440u0430u043cu043eu0442u0440u0438u0446u0430u0442u0435u043bu044cu043du044bu0445 organisms in the following quantities in the environment: from 0.55 to u0442u0430u043bu0438u00a0 acetate 1.6 g / l, u043du0430u043bu0438u0434u0438u043au0441u043eu0432u0430u00a0 acid from 0.005 to 0030 g / l.;4. u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0430u00a0 wednesday on p.1, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0 in that it contains u0440u0430u0437u0440u0443u0448u0430u0435u043cu044bu0435 u0445u0440u043eu043cu043eu0433u0435u043du043du044bu0435 u0444u043bu0443u043eu0440u043eu0433u0435u043du043du044bu0435 substance and at least one u0434u043bu00a0 identification of organisms chosen in the following the quantities in the environment: a u043du0438u0442u0440u043eu0444u0435u043du0438u043bu0444u043eu0441u0444u0430u0442u043du0430u00a0 salt u0434u0438u043du0430u0442u0440u0438u00a0 (pnp) from 0.2 to 0.8 g / l; 5 - bromine - 4 - chlorine - 3 - u0438u043du0434u043eu043bu0438u043b - d - u0433u043bu044eu043au043eu043fu0438u0440u0430u043du043eu0437u0438u0434 (x - glu), 4 - u043cu0435u0442u0438u043bu0443u043cu0431u0435u043bu043bu0438u0444u0435u0440u0438u043b - - d - u0433u043bu044eu043au043eu043fu0438u0440u0430u043du043eu0437u0438u0434 (mu - glu) 6 - chlorine - 3 - u0438u043du0434u043eu043bu0438u043b - - d - u0433u043bu044eu043au043eu043fu0438u0440u0430u043du043eu0437u0438u0434 (salmon - glu) of 0.05 to 0.4 g / l; u0446u0438u043au043bu043eu0433u0435u043au0441u0438u043bu0430u043cu043cu043eu043du0438u0435u0432u0430u00a0 salt 5 - bromine - 4 - chlorine - 3 - u0438u043du0434u043eu043bu0438u043b - - d - u0433u043bu044eu043au0443u0440u043eu043du0438u0434u0430 (x - gluc), u0446u0438u043au043bu043eu0433u0435u043au0441u0438u043bu0430u043cu043cu043eu043du0438u0435u0432u0430u00a0 salt 5 - bromine - 6 - chlorine - 3 - u0438u043du0434u043eu043bu0438u043b - - d - u0433u043bu044eu043au0443u0440u043eu043du0438u0434u0430 (magenta - Gluc) and u0442u0440u0438u0433u0438u0434u0440u0430u0442 4 - u043cu0435u0442u0438u043bu0443u043cu0431u0435u043bu043bu0438u0444u0435u0440u0438u043b - - d - u0433u043bu044eu043au0443u0440u043eu043du0438u0434u0430 (MUG) from 0.05 to 0.2 g / l.;5. u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0430u00a0 environment 1, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0 in that it contains u0441u043eu0435u0434u0438u043du0435u043du0438u00a0, u00a0u0432u043bu00a0u044eu0449u0438u0435u0441u00a0 indicators u0434u0435u0437u0430u043cu0438u043du0430u0437u043du043eu0439 activity in the following quantities in the environment: salt etc. u0440u0438u0432u0430u043bu0435u043du0442u043du044bu0445 metals, preferably iron citrate u0430u043cu043cu043eu043du0438u00a0 of from 0.5 to 1.0 g / l, the aromatic amino acids, preferably phenylalanine and tryptophan, to u043bu0438u0447u0435u0441u0442u0432u0435 from 1 to 2 g / l.;6. u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0430u00a0 environment 1, u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0 structure u0445u0440u043eu043cu043eu0433u0435u043du043du044bu0445 and u0444u043bu0443u043eu0440u043eu0433u0435u043du043du044bu0445 substances mixture from at least u0434u043bu00a0 enzyme for each species such u043au043eu043cu0431u0438u043d u0430u0446u0438u00a0u0445, which provide u043fu043eu00a0u0432u043bu0435u043du0438u0435 dyeing or different u0444u043bu0443u043eu0440u0435u0441u0446u0435u043du0446u0438u0438 u0434u043bu00a0 each villa as specified below;pnp + x - glu + magenta - gluc or MUG,;pnp + salmon - glu + x - gluc or MUG,;pnp + mu - glu + magenta - gluc or x - gluc.;7. u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0430u00a0 environment u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0 1, so that it further contains the agar with a density of from 400 to 700 g / cm2 in the number from 10 to 14 g / l u0434u043bu00a0 u043fu0440u0438u0433u043eu0442u043eu0432u043bu0435u043du0438u00a0 in tv u0435u0440u0434u043eu0439 form.;8. u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0430u00a0 environment u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0 1, so that the importance of u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 ph from 7 to 7.4.;9. u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du0430u00a0 environment u043eu0442u043bu0438u0447u0430u044eu0449u0430u00a0u0441u00a0 1, so that its u0433u043eu0442u043eu0432u00a0u0442 adding distilled water or u0434u0435u0438u043eu043du0438u0437u043eu0432u0430u043du043du043eu0439 to u043fu043eu043bu0443u0447u0435u043du0438u00a0 concentration from 25 to 80 g / l.