BACTERIUM STRAIN SPHINGOBACTERIUM MIZUTAE-32 AS PRODUCER OF SPML RESTRICTION ENDONUCLEASE, RECOGNIZING AND CLEAVING 5'-AT'CGAT-3' NUCLEOTIDE SEQUENCE

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to Bacterium strain sphingobacterium Mizutae-32 producing restriction endonuclease named as Spml, which recognizes and cleaves 5'-AT'CGAT-3' nucleotide sequence. Strain is isolated from melt water in process of searching endonuclease producing strain with high activity at low temperatures. Strain is capable of growth at 6°C, is stable for two week storage at 20°C. Strain-produced enzyme hydrolyzes phage-lambda DNA and T7 at 6-10°C. Enzyme yield is 300000 U/10 g of cells.;EFFECT: effective strain for restriction endonuclease production.;2 dwg, 1 tbl, 4 ex