SORBED AND INACTIVATED VACCINE AGAINST CATTLE INFECTIOUS RHINOTRACHEITIS

摘要

FIELD: veterinary virology, veterinary science, biotechnology.;SUBSTANCE: vaccine comprises antigenic material from the strain "VNIIZZH-DEP" of the cattle infectious rhinotracheitis virus reproduced in transplanted cells culture taken among transplanted cells BHK-21, MDBK and ZKG with the biological activity value at least 6.0 lg TCD₅₀/cm³ and inactivated with aminoethylethyleneimine and also aluminum hydroxide as an adjuvant and, additionally, saponin taken in the effective ratios. The strain is deposited in the collection VGNKI at the registration code "VNIIZZH-DEP". Virus of the strain "VNIIZZH-DEP" is reproduced in transplanted cultures of cells BHK-21, MDBK and ZKG inducing cytopathic effect and virus is accumulated with the titer value 6.5-8.0 lg TCD₅₀/cm³ for 48-96 h. Aminoethylethyleneimine is used in the concentration 0.1-0.2%, aluminum hydroxide is used as 3-4% gel in the amount 19.6-49.6 wt.-% and saponin is used as 10% aqueous solution in the amount 0.4 wt.-%. Vaccine shows high antigenic and immunogenic activity and harmless.;EFFECT: valuable properties of vaccine.;18 cl, 8 tbl, 8 ex