methods to study the interaction of substance and hormonrezeptor

摘要

A method for testing a substance interacting with a hormone receptor, characterized by comprising (a) maintaining a hormone receptor protein labeled with a marker substance capable of emitting an optical signal and a test substance, under predetermined conditions where a natural ligand is allowed to be bound to the receptor protein and where the receptor protein bound the natural ligand causes a change in its state, thereby inducing a change in a detectable characteristic of the optical signal emitted from the marker substance, (b) detecting the optical signal emitted form the marker substance under the predetermined conditions, and (c) comparing the characteristic of the optical signal detected in the step (b) with that of the optical signal detected from the marker substance in the absence of the test substance, thereby determining whether or not the test substance is a substance capable of interacting with the hormone receptor protein on the basis of the difference in both characteristics detected. IMAGE