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expression of starch binding domains and / or starch binding domains containing fusionsproteinen in plants

机译:淀粉融合域和/或含有融合蛋白的淀粉结合域在植物中的表达

摘要

The invention relates to a method for expressing a desired protein or polypeptide in a plant, in which the protein or polypeptide is expressed as a fusion with at least one starch binding domain. The plant is preferably a plant that contains or produces starch or starch granules in at least one of its parts, such as potato, sweet potato, cassava, pea, taro, sago, yam, banana and/or cereals such as rice, maize, wheat and barley. The protein or polypeptide can be an enzyme, in particular an enzyme that can convert, modify, alter, degrade or otherwise influence starch (granules); or can be a receptor or a structural protein. The invention further relates to the fusions thus obtained, to genetic constructs that encode the above fusions and to plants transformed with said constructs. The method of the invention can in particular be used to provide modified starches and/or to provide complexes of starch (granules) and the above fusions. In another embodiment, one or more starch binding domains are expressed in a plant, to provide a plant producing modified starches.
机译:本发明涉及在植物中表达所需蛋白质或多肽的方法,其中该蛋白质或多肽表达为与至少一个淀粉结合结构域的融合体。所述植物优选是在其至少一部分中包含或产生淀粉或淀粉颗粒的植物,例如马铃薯,红薯,木薯,豌豆,芋头,西米,山药,香蕉和/或谷物,例如大米,玉米,小麦和大麦。蛋白质或多肽可以是酶,特别是可以转化,修饰,改变,降解或以其他方式影响淀粉(颗粒)的酶。或可以是受体或结构蛋白。本发明进一步涉及由此获得的融合体,编码上述融合体的遗传构建体以及用所述构建体转化的植物。本发明的方法尤其可以用于提供改性淀粉和/或提供淀粉(颗粒)和上述融合物的复合物。在另一个实施方案中,一个或多个淀粉结合结构域在植物中表达,以提供产生改性淀粉的植物。

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