agglutinationsassay for the detection of e. coli

摘要

A method for detecting Escherichia coli in a sample by providing a sample suspected of containing Escherichia coli; contacting the sample with beads coated with a mixture of antibodies or anti-serum specific to one or more surface antigens of Escherichia coli, each of the one or more surface antigens having a molecular weight of 21 ± 2 KDa, 26 ± 2 KDa, 31 ± 2 KDa, 36 ± 2 KDa, 38 ± 2 KDa, 67 ± 2 KDa, and 77.8 ± 2 KDa; and observing agglutination of the beads, where the presence of the agglutination indicates the presence of Escherichia coli in the sample.