首页> 外国专利> An immunoassay for human chromogranins a (cga), antibodies, reagent and for this purpose a suitable test kit

An immunoassay for human chromogranins a (cga), antibodies, reagent and for this purpose a suitable test kit

机译:用于人类嗜铬粒蛋白a(cga),抗体,试剂的免疫测定法,为此目的,使用合适的检测试剂盒

摘要

The invention concerns the immunologic assay of human chromogranin A (CgA) and a kit useful for said assay. Said assay comprises a step for detecting said chromogranin A (CgA) by reacting at least one monoclonal or polyclonal antibody which specifically binds with an epitope located in the amino acid sequence ranging between amino acid sequence no 145 to 234 starting from the human CgA N-terminal end. The assay can be carried out by competition with marked CgA or by the sandwich method using two antibodies, for instance the amino acid sequence 219-234 and the marked sequence 145-197.
机译:本发明涉及人嗜铬粒蛋白A(CgA)的免疫学测定和用于所述测定的试剂盒。所述测定法包括通过使至少一种单克隆或多克隆抗体与所述嗜铬粒蛋白A(CgA)反应的步骤,所述单克隆抗体或多克隆抗体与位于从人CgA N-开始的第145至234位氨基酸序列之间的氨基酸序列中的表位特异性结合终端。该测定可以通过与标记的CgA竞争或通过使用两种抗体例如氨基酸序列219-234和标记的序列145-197的夹心法进行。

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