synthetic of culture medium and / or storage and / or embryo transfer or cells.

摘要

The invention relates to a synthetic medium of the culture and / or of freezing and / or storage and / or transfer of cells or embryos, human or animal origin, this medium in the form of a solution comprising a basic medium free of natural proteins of animal or human origin, the basic components of this basic medium including essentially salts of calcium, sodium and potassium, at least one amino acid or an amino acid derivative, preferably of vitamins, and optionally at least one antibiotique.ce medium contains, in addition to the basic medium, at least a substitute for the natural proteins of animal or human origin, this substitute on the basis of recombinant molecules being constituted by the combination of the β1 tgf (transforming growth factor β1) and igf - i (insulin like growth factor i), with at least a growth factor or cytokine chosen from the group constituted by the lif (leukemia inhibitory factor), the gm - ch (granulocyte colony stimulating factor) and macrophage bfgf (basic fibroblast growth factor).