METHOD FOR PURIFYING PRECURSOR CELL OF FOLLICULAR DENDRITIC CELL AND CONSTRUCTION OF LYMPHATIC DEVICE USING THE SAME

摘要

PROBLEM TO BE SOLVED: To provide a precursor cell of a follicular dendritic cell and a lymph node like structure.;SOLUTION: This method for separating the precursor cell of the follicular dendritic cell is provided by separating a negative cell fraction by a negative selection using antibodies against cell surface markers CD4, CD3, CD19, CD11c, CD11b, F4/80, Gr-1 and CD8 and fractionally collecting weakly attached cells from a secondary lymphatic tissue-derived cell dispersion to separate the weakly attaching cell fraction having a negative marker and further separating a positive cell fraction from that cell fraction by a positive selection by using an antibody against the cell surface marker B220, The precursor cells of the follicular dendritic cells obtained by the method, and a method for preparing the lymph node like structure obtained by hypodermically administering the precursor cells of follicular dendritic cell to a non-human animals are also provided.;COPYRIGHT: (C)2007,JPO&INPIT