Amplification reactions and ligation reactions to determine the target molecule

摘要

The present invention relates to a kit methods, and reagents for the (or quantifying) detecting the presence or absence of the protein and the polynucleotide sequence of the target in at least one sample using the response code and decoding reactions. For example, when a particular target polynucleotide is present in the sample, a reaction product comprising an addressable primer portion is formed in the encoding reaction. Obtained, so that, depending on whether the absent sequence exists or address one primer used in the decoding amplification reaction and at least labeling probe one, it may provide a detectable signal value of at least . In some embodiments, the encoding is analyzed single nucleotide polymorphisms (SNP) and includes a ligation reaction with the linker probe.