METHOD FOR PRODUCING SINGLE STRAND GENE TAG GROUP CONTAINING TRANSCRIPTION STARTING SITE

摘要

PPROBLEM TO BE SOLVED: To provide a method for producing a single strand gene tag group reflective of a kind and a ratio of quantity of a base sequence group at the mRNA 5' terminal extracted from a eukaryotic cell, to provide a method for measuring an expression level of a gene in the eukaryotic cell including a process for hybridizing a solid phase on which a DNA or a RNA containing a transcription starting site is immobilized with the single strand gene tag group, and to provide a method for preparing a gene expression profile by integrating obtained information on gene expression. PSOLUTION: This single strand gene tag group reflective of the kind and the ratio of the quantity of the base sequence at the mRNA 5' terminal is produced by combining a method disclosed in a 5'SAGE method with a procedure for forming a double-stranded DNA into a single strand. Thus, it is found that the single strand gene tag group is effective for recognizing expression of a gene which targets an expression starting site, as a result of conducting hybridization with a DNA chip by using the tag group as a sample. Further, utilization of the single strand gene tag group makes it possible to conduct comprehensive expression analysis of a gene which targets various transcription starting sites. PCOPYRIGHT: (C)2007,JPO&INPIT