Environment to maintain the function of primary hepatocytes

摘要

Surfaces useful for cell culture, such as collagen VI and support it binds CAR material, elastin, which are bound to the CAR material, fibronectin, vitronectin, laminin, collagen I, collagen III, collagen IV, and I and a fragment or variant thereof or a biologically active ECM proteins. In addition, the active agent, preferably polyethylene imine (PEI), poly -D- lysine (PDL), poly -L- lysine (PLL), poly-poly -L- -D- ornithine or ornithine (PDO) as (PLO) fragment or variant thereof or a biologically active polycationic polymers are present on the surface, optionally. This surface is used for cell culture, to promote the proliferation and / or cell adhesion of primary hepatocytes, survival, and maintenance of function. As a method for the proliferation and / or cell adhesion, survival and maintenance of function, the present invention is also directed to a method of using this surface. It further discloses the use of the surface of the cell cultured with serum-free medium. I also disclosed screening method that uses the surface of the present invention.