Immunization and immune activity measurement method

摘要

PROBLEM TO BE SOLVED: To immunize a lymphocyte efficiently and to obtain an antigen specific antibody at high concentration by adding an antigen to a culture liquid combining an adjuvant substance and cytokine in order to culture lymphocyte and then measuring immunological activity. ;SOLUTION: When lymphocyte coexists with an antigen in a culture liquid containing a combination of an adjuvant substance and cytokine, lymphocyte can be immunize specifically with high efficiency and a large quantity of antigen speciftc antibody can be obtained. Muramyldipeptide is employed as an adjuvant substance in the lymphocyte. One kind selected from a group of interleukin 2, 4, 6 is employed as the cytokine. Dulbecco MEM medium, Ham F12 medium, or the like, 15 employed as the culture liquid. Keyhole lympet hemocyanin, cholera toxin B subunit, or the like, is employed as the antigen. Lymphocyte in organism or blood (e.g. peripheral blood) is employed as the lymphocyte.;COPYRIGHT: (C)1998,JPO