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Lysis method and gene amplification or detection method using the same acid-fast bacilli
Lysis method and gene amplification or detection method using the same acid-fast bacilli
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机译:使用相同的抗酸杆菌的裂解方法和基因扩增或检测方法
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摘要
A method of effecting lysis of acid-fast bacteria, comprising heating acid-fast bacteria in a liquid containing a non-ionic surfactant at a temperature of below the boiling point of the liquid. This method enables accomplishing secure lysis of acid-fast bacteria in a simple manner within a short period of time without the use of special apparatus and agent and enables extracting genes. The heating is preferably conducted at 96 DEG C for 10 min. As the nonionic surfactant use can be made of a d-sorbitol fatty acid ester, a polyoxyethylene glycol sorbitan alkyl ester, a polyoxyethylene glycol p-t-octylphenyl ether or the like. The pH value of the liquid is preferably 8, and the liquid preferably contains EDTA. It is also preferred that before the heating, the acid-fast bacteria be treated with lipase.
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