Production method of 2,6-dihydroxybenzoic acid and 2,6-dihydroxybenzoic acid decarboxylase having heat resistance

摘要

The object of the present invention to provide an advantageous method industrially to produce the 2,6-dihydroxybenzoic acid are contacted in an aqueous medium containing carbon dioxide and carbonate ions and / or resorcinol and processed product of the microorganism or microorganisms I is that. I find an enzyme with high heat resistance capable of producing 2,6-dihydroxybenzoic acid from resorcinol from a microorganism belonging to the (Rhizobium) genus Rhizobium. I to obtain a transformant microorganism transformed with a gene encoding the enzyme. I to produce 2,6-dihydroxybenzoic acid is reacted to resorcinol in an aqueous medium in the presence of carbon dioxide and carbonate ions and / or the enzyme produced by said transformed microorganism. Also, when producing 2,6-dihydroxybenzoic acid and resorcinol in an aqueous medium containing carbon dioxide and carbonate ions and / or by using the processed product of the microorganism or microorganisms, are added to the aqueous solvent and the organic solvent, resorcinol to suppress oxidation, or to improve the storage density by sequential addition of one processed product of the microorganism resorcinol, or microorganisms. It is possible to obtain a better result using the transformed microorganism of the above case. The above manufacturing method can be applied to the production using catechol 2,3-dihydroxybenzoic acid.