New phytase and the new phytase which possesses

摘要

PROBLEM TO BE SOLVED: To obtain a new phytase useful in the field of feed and food industries, excellent in stability, and capable of improving effects, such as prevention of environmental pollution, by having specific physicochemical properties. ;SOLUTION: This phytase has the following physicochemical properties: Action; it hydrolyzes phytic acid to produce myoinositol and free phosphate. Substrate specificity; it stronly acts on phytic acid, and also acts on various phosphate esters. Acting pH and optimum pH; it shows activity at pH 2-6 and optimum pH is 4-5. pH stability; it is stable at 2.5-6.5 at 37°C for 1 hr. Temperature; it is active at a temp. of 30-60°C and optimum teperature is 55°C. Temperature stability; it is stable up to 55°C, when incubated at pH 5.5 for 30 min. Molecular weight; it has molecular weight of 130-150 kDa by gel filtration, Isoelectric point; it has isoelectric point of 4.4. This new phytase is obtained by culturing a new microbial strain (FER M P-15744) belonging to the genus Penicillium, or its natural or artificial mutant.;COPYRIGHT: (C)1999,JPO