Size and/or growth engineering by modulation of the interaction between calmodulin, and brassinosteroid biosynthetic enzymes and orthologs therof

摘要

Particular aspects show that DWF1 is a Ca²⁺/calmodulin-binding protein, and this binding is critical for function. Molecular/genetic analysis using site-directed and deletion mutants revealed that loss of calmodulin binding abolished the function of DWF1 in planta, whereas partial loss of calmodulin binding resulted in a partial dwarf phenotype in complementation studies, providing direct proof that Ca²⁺/calmodulin-mediated signalling has a critical role in controlling the DWF1 function. Furthermore, DWF1 orthologues from other plants have a similar Ca²⁺/calmodulin-binding domain, indicating that Ca²⁺/calmodulin regulation of DWF1 and its homologues is common, and broadly applicable in plants. Methods for generating size-engineered crops are provided by altering the Ca²⁺/calmodulin-binding property of their DWF1 orthologues. Likewise, according to additional aspects, DWF4, DWF5 and CPD were also shown to be Ca²⁺/calmodulin-binding proteins, and can likewise be used for generating size-engineered crops by altering the Ca²⁺/calmodulin-binding property of their orthologues in a variety of plants, including crop plants.