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Method of producing lens cell and lens cell obtained by the method

机译:晶状体晶胞的制造方法以及通过该方法得到的晶状体晶胞

摘要

A method of producing a lens cell is disclosed. The method includes: an ES cell maintenance step of maintaining an ES cell by using a medium containing a fibroblast growth factor FGF-2 at a concentration of 2 ng/ml to 50 ng/ml; and a differentiation inducing step, carried out after the ES cell maintenance step, of inducing differentiation of the ES cell into a lens cell by implanting and culturing the ES cell on a mouse-skull-cell PA6 at a cell density of 2 colonies/cm2 to 6.5 colonies/cm2. In one embodiment, a washing step, carried out between the ES cell maintenance step and the differentiation inducing step, of washing the maintained ES cell once with an ES differentiation medium is included.
机译:公开了一种生产晶状体细胞的方法。该方法包括:ES细胞维持步骤,其通过使用浓度为2ng / ml至50ng / ml的成纤维细胞生长因子FGF-2的培养基来维持ES细胞。在ES细胞维持步骤之后进行的分化诱导步骤,其通过以2个菌落/ cm 2的细胞密度将ES细胞植入并培养在小鼠头骨细胞PA6上来诱导ES细胞分化为晶状细胞 2 至6.5菌落/ cm 2 。在一个实施方案中,包括在ES细胞维持步骤和分化诱导步骤之间进行的洗涤步骤,其用ES分化培养基洗涤维持的ES细胞一次。

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