Multiplexed RT-PCR assay for detection and separation of barley and cereal yellow dwarf viruses

摘要

RT-PCR primer multiplexes and a method for detecting the presence of Subgroup I and II barley or cereal yellow dwarf viruses in a sample. A first multiplex includes a first primer pair that selectively amplifies Subgroup I viruses, and a second primer pair that selectively amplifies Subgroup II viruses. A second multiplex includes primer pairs that also selectively amplify Subgroup I BYDV-PAV, BYDV-MAV, and BYDV-SGV. The PCR fragments resulting from the multiplexes differ in size. Therefore, with the first multiplex, a sample can be identified as containing either a Subgroup I or a Subgroup II barley or cereal yellow dwarf virus, and with the second multiplex, specific Subgroup I virus in the sample also can be identified. The present invention also relates to a kit including these RT-PCR primer multiplexes.