Method of posttranslational modification by adding mycrosomal membrane in cell-free protein synthesis

摘要

It is intended to provide a novel method for posttranslational modification in cell-free protein synthesis and a novel method for cell-free protein synthesis with the use of such posttranslational modification reaction. A method for synthesizing a protein in a cell-free system using an extract liquid for cell-free protein synthesis containing an arthropod-derived extract, characterized in that translation reaction is carried out in the presence of mammal-derived microsomal membranes.