Method for cell-free synthesizing protein using wheat embryo extract under low temperature condition

摘要

The subject of the present invention is to provide a method for cell-free protein synthesis which needs no new apparatus and reagent to allow production of a protein with a high quality by a large amount. To solve the matter described above, translation reaction is conducted at a temperature of 0° C.-20° C., which is lower than approximately 26° C., that is, a temperature suitable for a conventionally known method for cell-free protein synthesis using a wheat embryo extract.