Method of measuring lactate dehydrogenase activity in serum, and device for measuring lactate dehydrogenase activity in serum

摘要

A new electrochemical measurement method is provided that is excellent in accuracy in detecting LDH activity in a serum sample and shortens the time required for the detection. The method of measuring lactate dehydrogenase activity in serum includes: a first step (S1) of adding at least lactic acid, a coenzyme of a lactate dehydrogenase, and an electron mediator to a first serum sample that contains the lactate dehydrogenase to prepare a second serum sample; a second step (S2) of removing molecules whose molecular weights are 30000 or higher, preferably molecules whose molecular weights are 10000 or higher from the second serum sample to prepare a third serum sample; and a third step (S3) of measuring a value of current that is generated by applying voltage to the third serum sample.