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Serial analysis of ribosomal and other microbial sequence tags
Serial analysis of ribosomal and other microbial sequence tags
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机译:核糖体和其他微生物序列标签的系列分析
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摘要
A simple and robust method for genetic analysis of complex microbial communities involves the steps of PCR amplification of V1 region of rrs genes in the community DNA sample using two universal primers, followed by cleavage by BsgI and removal of dual-biotinylated primers using streptavidin-coated magnetic beads to purify RSTs, and concatemerization of the RSTs and size selection of resultant concatemers by agarose gel electrophoresis. The isolated concatamers are then cloned and sequenced and subjected to sequence analyses to enable identification of the members of the microbial community.
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