Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis

摘要

Methods for catalyzing cleavage of a bond with an electric field device. In one method, a catalytic peptide having a first reactive group and a second reactive group is coupled to an electrode. The catalytic peptide is then contacted with a solution containing a substrate. The first reactive group reacts with the substrate to form a first intermediate. The second reactive group then reacts with the first intermediate to form a positively-charged second intermediate having an acyl bond and a negatively-charged first reactive group. An electronic pulsing sequence is then applied to the electrode to separate the negatively-charged first reactive group and the positively-charged second intermediate. The second intermediate is then reacted by acyl transfer to cleave the acyl bond. The first reactive group may be a sulfhydryl or deprotonated sulfhydryl. The second reactive group may be an imidazole. The substrate may contain an ester or amide bond.