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Insertion of furin protease cleavage sites in membrane proteins and uses thereof
Insertion of furin protease cleavage sites in membrane proteins and uses thereof
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机译:弗林蛋白酶蛋白酶切割位点在膜蛋白中的插入及其用途
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摘要
Cleavage site for the protease furin is inserted between domains of a membrane glycoprotein. Upon cleavage by furin in the trans-Golgi network, the protein is separated into individual membrane-free domain that retains its native conformation. This protocol can be used to produce virus membrane protein domains for structural analysis and for trials as vaccines.
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