Use of a fluorescent protein for detecting interactions between a target protein and its ligand

摘要

A fluorescent protein selected in particular among the autofluorescent proteins for detecting the non-covalent interaction between a target protein marked by the fluorescent protein and one of its ligands marked by a marker consisting of: either a molecule capable of absorbing the light emitted by the fluorescent protein, or a fluorescent substance, the detection taking place by fluorescence energy transfer: between the fluorescent protein and the fluorescent substance, the fluorescent substance being such that it is excitable at the fluorescent protein emitting wavelength, or it emits at the fluorescent protein emitting wavelength; between the fluorescent protein and the molecule capable of absorbing the light emitted by the fluorescent protein.