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Methodology to obtain neutral salts soluble Nucleic Acids from Fresh glands, organs, tissues, for Therapeutic application in different diseases

机译:从新鲜腺体,器官,组织中获得中性盐可溶核酸的方法学,用于不同疾病的治疗应用

摘要

This to pharmacodynamics of ribonucleic acids for Therapeutic application in the most diverse Pathology.Claim 1: a procedure to obtain neutral salts soluble Nucleic Acids, tissues, or organs Fresh glands of mammals, including Cattle, Pigs or Sheep.The type which comprises treating the materials previously homogenized with a Hypertonic solution of Sodium Chloride in maceration; Keep the mixture at a temperature not exceeding 10sc; heat the Mash to bathroom Maria during a period of time sufficient to coagulate Proteins Wherein the Mash is allowed to cool to a temperature below 40sc,And then there is a choice of the ketone group formed by acetone and Benzophenone, metu00edlotiloetona,Incorporated in a relationship between 20 and 50% by Weight with respect to the mass of mash; Separating insoluble solids and recover the aqueous fraction to precipitate the Nucleic Acids with + 3; separating the Precipitated nucleic acids and salts of + 3 and its resolubilizarlos Pendiu00e9ndolas in an aqueous medium of pH greater than 7 and Recover aqueous fraction containing acid nUcleicos Solubilized Salts and a disposable fraction containing LANTHANUM precipitate.
机译:权利要求1:一种获得中性盐可溶的核酸,组织或器官的方法,该方法包括哺乳动物的新鲜腺体,包括牛,猪或绵羊。预先在浸渍中用高渗氯化钠溶液均质的材料;保持混合物温度不超过10sc;在足以使蛋白质凝结的时间内将Mash加热至浴室Maria,其中将Mash冷却至40sc以下的温度,然后可以选择由丙酮和二苯甲酮形成的酮基,二甲基苯并噻吩并入相对于糊的质量为20至50%(重量)之间的关系;分离不溶的固体并回收含水部分,以+3沉淀出核酸。在pH大于7的水性介质中分离+ 3的沉淀的核酸和盐及其可再溶解的盐,并回收含酸性核酸溶解盐的水部分和含LANTHANUM的可弃部分。

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