A polypeptide in a way that hexose oxidase activity and isolated a recombinant DNA Molecule

摘要

A polypeptide in an isolated form having hexose oxidase activity, comprising at least one amino acid sequence selected from the group consisting of: (i) Tyr-Glu-Pro-Tyr-Gly-Gly-Val-Pro, (ii) Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val- Glu-Ser-Gly-Tyr-Asp-XXX-Gly-Tyr-X-Val-Ser-Ser, (iii) Asp-Leu-Pro -Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser-Asn-Leu-X-Phe, (iv) Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe- X-Ala-His-Thr, (v) Tyr-Tyr-Phe-Lys, (vi) Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala- Gly-Thr-X- Asp, (vii) Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp, (viii) X-Ile-Arg-Asp-Phe-Tyr-Glu-Glu-Met, where X represents an amino acid selected from the group consisting of Ala, Arg,Asn Asp Asx Cys Gln Glu Glx Gly His Ile Leu Lys Met Phe Pro Ser Thr Trp Tyr y Val y en donde dicho polip ptido es un polip ptido de fusion Una mol cula de DNA recombinante que comprende un fragmento de DNA que codifica un polip ptido (v) X represents a specific aminobenzene selected from glu-glu-glu-met, and a specific GLX, Gly, his, ile, Leu, Lys, met, Phe, pro, Ser selected from aminobenzoic acid selected from the following substances, THR, Trp, Tyr and Val, where DNA fragments are operatively linked to a promotional sequence,In this driver there is no intrinsic connection with the DNA fragment. The appropriate sources for encoding DNA into hexosa oxidase active peptides are algae species, including Chondrus crispus, iridphycus flaidum and eutima crista. In practical forms, the active peptide hexosa oxidase is produced by Pichia pastoris, cervasiae Sacharomyces or E.coli.