首页>
外国专利>
METHOD FOR THE PRODUCTION OF TRANSGENIC PLANTS USING APICAL SHOOT TIPS
METHOD FOR THE PRODUCTION OF TRANSGENIC PLANTS USING APICAL SHOOT TIPS
展开▼
机译:利用API SHOT TIPS生产转基因植物的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
This application provides a method for transformation of cells of plant tissues and the regeneration of these cells and tissues into mature transgenic plants. The apical meristematic region of a plant seedling are isolated, then treated to slow the metabolic activity of the isolated tissue. The tissue is then exposed to a transforming agent such as a DNA plasmid or an Agrobacterium carrying a plasmid vector which contains a DNA encoding a gene of interest. After an appropriate incubation period, the treatment is reversed, allowing the metabolic activity of the apical meristematic tissues to return to normal. The method provides a larger population of receptive cells for exposure to the transforming agents, thereby providing higher probabilities of isolating transformed tissues which can be regenerated into transformed plants.
展开▼