METHOD FOR GENERATING NEURAL PRECURSOR CELLS, NON-TUMORIGENIC CELL COMPOSITION CONTAINING THEM AND USES THEREOF IN PREPARATION OF PHARMACEUTICAL COMPOSITIONS

摘要

A process for producing neuronal or glial progenitor cells, comprising: (a) culturing embryonic stem cells to form embryoid bodies; (b) culturing the embryoid bodies to form neural progenitor cells; (c) proliferating the neural progenitor cells in a serum-free medium containing growth factor(s); (d) further proliferating the neural progenitor cells in a serum-free medium containing growth factor(s) and isolating the neural progenitor cells; and (e) further proliferating the neural progenitor cells in a serum-free medium containing growth factor(s) and isolating the neural progenitor cells. Independent claims are included for the following: (1) a process for producing glial progenitor cells, comprising: (a) culturing embryonic stem cells to form embryoid bodies; (b) culturing the embryoid bodies to form neural progenitor cells; (c) proliferating the neural progenitor cells in a serum-free medium containing growth factor(s); (d) further proliferating the neural progenitor cells in a serum-free medium containing growth factor(s) form spheroids with neuronal and glial differentiation potential; and (e) proliferating the spheroids in a serum-free medium containing growth factor(s) until a lawn of glial progenitor cells is formed, and isolating the cells; (2) neuronal or glial progenitor cells that are produced as above and include no more than 15% of primitive embryonic cells and non-neural cells; and (3) a cell library comprising autologous and nonautologous cells as above.