A method for reducing motility of mammalian spermatozoa and reversing the same, said method comprising. (i) mixing a composition comprising (a) sodium hydrogen phosphate buffer having disodium hydrogen phosphate (Na2HPC4) ranging between 0.23 - 0.27 gms and sodium dihydrogen phosphate (NaFPC.FO) ranging between 0.023-0.027 gms, (b) sodium chloride ranging between 0.8 to 1.0 gms, and (c) 30% hydrogen peroxide ranging between 6.5 - 8.0 ml, and adding water to make up total volume to 100ml,with pH of said composition ranging between 7.0 to 7.4, and semen containing mammalian spermatozoa, (ii) incubating the said mixture for a period ranging between 5-15 minutes, (iii) obtaining the mammalian spermatozoa with reduced motility in the said mixture, (iv) incubating the mammalian spermatozoa with reduced motility in the mixture for at least another 45-55 minutes, and (v) obtaining mammalian spermatozoa with normal motility.
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