EXTRACTION OF RECOMBINANT PROTEINS FROM INCLUSION BODIES USING A DENATURANT SOLUTION CONTAINING AN ORGANIC SOLVENT

摘要

- solubilizing recombinant proteins expressed in the said E.coli with a buffer having a pH in the range of 7 to 11, wherein the said buffer contains guanidine hydrochloride, organic solvent and water, extracting the protein at temperatures ranging from 15°C to 40°C.