DOUBLE DYE METHOD TO IN ACTIVATE BLOOD BORNE PATHOGENS

摘要

Treatment of blood and blood fractions with a mixture of rnethylene blue and crystal violet ("Double-Dye") has been found to eliminate a variety of bacteria, virus and parasites without appreciably damaging blood cells or proteins. A 4-log reduction in Staphylococcus epidermidis, Staphylococcus aureits, Bacillus subtilis, Escherichia coli, Yersinia enterocolitica, Pseudomonas aeruginosa, Pseudomonas fluorescent and Serratia marcescens was observed. Complete kills of vesicular stomatitis virus, encephalomyocarditis virus, bovine diarrheal virus and porcine parvovirus were achieved by adding sufficient ascorbate to largely convert the two dyes into their leuco forms. Finally, a PVA filtration method is used to remove the dyes before use of the disinfected blood or blood component. Thus, the Double-Dye-ascorbate-PVA method is an effective, low-cost way to eliminate pathogens from whole blood, platelets, plasma or other blood fractions