HIGHLY SENSITIVE HOMOGENEOUS ASSAY BASED ON ANTI-STOKES SHIFT FRET MEASUREMENT

摘要

This invention relates to a novel time-resolved lanthanide- based energy transfer assay utilizing non-overlapping acceptor fluorophores, having their absorption maximum energetically at higher level than the main emittive energy level of the donor. In this assay the lifetime of the energy transfer enhanced acceptor signal is partially independent of the energy transfer rate. The use of non-overlapping acceptors enables an anti-Stokes' shift FRET measure- ment, in which the acceptor emission is created and measured at a shorter wavelength than the actual donor emission.