A PROCESS FOR PREPARING A NOVEL BIOFERTILISER STRAINS OF NITROGEN FIXER AND PHOSPHATE SOLUBILISER TO PROMOTE SOIL HEALTH, IN PARTICULAR FOR GROWTH OF TEA PLANTS'.

摘要

This invention relates to a process for preparing a novel biofertiliser strains of nitrogen fixer and phosphate solubiliser to promote soil health in particular for growth of tea plants comprising of nitrogen fixer and phosphate solubiliser in equal proportion wherein the nitrogen fixer is prepared by the steps of a) transferring the stock culture Azotobacter Chroococcum (BAH) into mother culture modified Ashley's medium, b) allowing the mother culture medium to grow for 3-7 days, c) transferring the grown mother culture to main culture medium and d) allowing the main culture medium to grow ge for another 3-7 days to obtain Azotobacter choococcum (BAH) strains and in which the phosphate solubiiiser is prepared from as a mixture of Bacillus subtilis (BB15), Pseudomonas Putida (BP19) Aspergillus awamori (FA16) and Aspergitlus flavus (FA18) in a ratio of 5:2:2:1 in Pikovskaya's medium wherein Bacillus Subtilis (BB15) and Pseudomonas putida (BP19) are prepared as in a manner of preparing Azotobacter Choococcum and Aspergillus awamori (FA16) and Aspergillus flavus (FA18) (molasses or yeast medium) are prepared by transferring the stock culture to mother culture medium, allowing the mother culture to grow for 15 days, transferring the grown mother culture to main culture medium and allowing it to grow again for another 15 days to obtain Aspergillus awamori (FA16) and Aspergillus flavus (FA18) strains.