GENETICALLY ENGINEERED PYRROLOQUINOLINE QUINONE DEPENDENT GLUCOSE DEHYDROGENASE COMPRISING AN AMINO ACID INSERTION.

摘要

The present invention relates to improved variants of soluble pyrroloquinoline quinone(PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertionbetween positions 428 and 429 as corresponding to the amino acid sequence knownfrom Acinetobacter calcoaceticus, to genes encoding such variant s iGDH,to proteins of such s-GDH variants with improved substrate specificity for glucose,and to different applications of these s-GDH variants, particularly for determiningconcentrations of sugars, especially of glucose in a sample.