There is provided a lateral flow assay device for detecting the presence or quantityof an analyte residing in a test sample where the lateral flow assay device hasa porous membrane in communication with a conjugate pad and a wicking pad. Theporous membrane has a detection zone which has an immobilized first capture reagentconfigured to bind to at least a portion of the analyte and analyte-conjugatecomplexes to generate a detection signal. A control zone may be located downstreamfrom the detection zone on the porous membrane and has a second capture reagentimmobilized within the control zone. The conjugate pad is located upstream fromthe detection zone, and has detection probes with specific binding members forthe analyte. The sample is deposited between the control and detection zones.A buffer release zone is located upstream of the conjugate pad and provides forbuffer addition to the device, the buffer serving to move the detection probesto the detection and control zones.
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