METHOD OF PRODUCING RECOMBINANT SUCROSE SYNTHASE, USE THEREOF IN THE PRODUCTION OF SUCROSE-DETERMINATION KITS, METHOD OF PRODUCING ADPGLUCOSE AND METHOD OF OBTAINING TRANSGENIC PLANTS HAVING LEAVES AND RESERVE ORGANS WHICH ACCUMULATE A HIGH CONCENTRA

摘要

The invention relates to a method for the efficient production of large quantities of soluble recombinant sucrose synthase (SS) in the active form thereof, with the expression of the gene that codes for SS in a strain of Escherichia coli. The expression vector used enables the recombinant SS thus produced to have a histidine tail which facilitates the rapid purification thereof. In addition, the invention relates to sequences of mutated versions of the SS gene, which code for SS isoforms that are suitable for the production of ADPG. The invention also relates to an efficient method for the production of ADPG and UDPG, using the wild and mutated versions of recombinant SS. The invention further relates to the use of SS for the production of sucrose determination test devices. Finally, the invention relates to a method of obtaining transgenic plants which overexpress the SS gene, either constituently or in the reserve organs or leaves thereof, and which have a high concentration (both in the leaves and in the reserve tissues) of sucrose, ADPG, G6P and starch owing to the high ADPG-synthesising activity of SS.