identification and molecular cloning of the gene (cdna) rd 22 in response to dehydration in the vineyard

摘要

a candidate gene approach was performed on banks by using pcr (polymerase chain reaction) has allowed us to isolate in grape variety cabernet sauvignon. the full-length cdna of rd. the whole fragment was obtained by a series of conventional and asymmetric polymerase chain reaction (pcr) using specific primers and universal internal.the pcr product was gel purified extract, and with the aid of a purification kit, cloned in the vector for sequencing pgemt easy, and then synthesized again by a high fidelity pcr performance and to validate the sequence, and then cloned into the binary vector over p green.agrobacterium tumefaciens gv3010 was transformed by electroporation to introduce the gene construct of the rd22.