METHOD FOR PRODUCING BIOLOGICAL PREPARATION BASED ON CELLS OF EMBRYONIC DOG'S LIVER FOR CORRECTING IMMUNOHEMATOPOIESIS IN DOGS

摘要

The method for producing the biological preparation based on the cells of the embryonic dog's liver for correcting immunohematopoiesis in the dogs comprises enzyme infiltration, mincing, disaggregation, filtration, centrifugation, washing and cryopreservation. The liver of 37-45-day embryos of dogs is used. The liver tissue is infiltrated with collagenase solution (50 mg of collagenase in 20 ml of Hanks solution). Then RPMI medium and cryoprotective medium are added.