Purification of fibrinogen from milk using cation exchange chromatography.

摘要

A method for obtaining fibrinogen from milk, comprising: a. Contacting the milk with a substrate of cation exchange chromatography under conditions wherein the substrate and the milk is at a pH greater than the pI value of fibrinogen and fibrinogen connections to the substrate; B. optionally washing the substrate to remove unbound components; and c. removal of the bound fibrinogen from the substrate by using irrigating means, wherein the irrigation means have an increased ionic strength or increased pH or both relative to the conditions in step (a).