USE OF NUCLEAR MAGNETIC RESONANCE 13C TO DETECT UNIONS.

摘要

A method for detecting the binding between a putative ligand and a preselected target molecule, enriched with 13C, comprising: a) generating a first two-dimensional 13C / 1H NMR correlation spectrum of said target molecule; b) forming a mixture of said target molecule with at least one putative ligand compound; c) generate a second two-dimensional 13C / 1H NMR correlation spectrum of the mixture of step b); and d) compare the first and second spectra; wherein said target molecule is prepared by culturing a transformed cell line, which contains an expression vector containing a polynucleotide encoding said target molecule, in a medium containing a biosynthetic precursor of an amino acid selected from the group consisting of 4-ketobutyric acid 4- (13C) and 4- ketomethylbutyric acid (13C) -3- (13C).