Method for the detection of minority genomes in viral cuasispecies using DNA microchips.

摘要

The method enables the detection of minority genomes, in particular minority memory genomes, a nucleic acid population of a viral quasispecies present in a population of less than 50%, containing at least one mutation in relation to the majority genome of this quasispecies, and comprising: a) extracting the nucleic acid of this viral quasispecies from a sample suspected to contain this viral quasispecies; b) amplifying at least one nucleic acid fragment of said viral species; c) detecting and analyzing the existence of minority genomes by using techniques based on DNA microchips. This method has application in genetic diagnosis of viral diseases.